Magrose Protein A Magbeads for Protein Purification 30-150 μm, 10%
(v/v)
1, Description:
BeaverBeads™ Protein A, Protein G are superparamagnetic beads
covalently coated with recombinant Protein A or Protein G that
contains Fc binding domains for the purification of monoclonal or
polyclonal antibodies from cell lysate, blood plasma, ascites and
tissue culture supernatant, and for antibody immobilization
studies. This product is micron magnetic beads, skilled operation
can complete the antibody adsorption process within 15 min, and
complete the process of antibody purification within 30 min. It can
be used for antibody fixation and other related studies. By
referring the attached Table 1 users can select the categories of
magnetic beads according to the source and subtype of the target
antibody, and compare the affinity of Magrose Protein A and Magrose
Protein G beads with different antibodies.
2, Product Information:
| Product Name | Magrose Protein A |
Bead Size | 30~150 μm |
Beads Concentration | 10% (v/v) |
Ligand | Protein A |
Medium | Magrose |
Antibody Bonding Quantity | 25~30 mg Human IgG/mL Gel |
Storage Temperature | 2~8℃ |
| Binding/Washing Buffer | PBST (pH 7.2~7.4): 137 mM NaCl,2.7 mM KCl, 10 mM Na2HPO4, 2.0 mM KH2PO4, 0.1% Tween-20 |
Elution buffer | 100 mM Gly, 0.1% Tween-20, pH 2.5 |
Neutrilization buffer | 1.0 M Tris-HCl, pH 9.0 |
Storage buffer | PBST (includes 0.05% NaN3) |
Shelf life | 12 months |
3, Feature:
1. Highly effective antibody binding capacity and ultra-low
non-specific adsorption performance
2. Time saving, simple and mild
3. High product stability
4. Antibody elution system is closer to neutral
5. Very low protein A ligand off rate
6. Reusable
4, FAQ:
Q1: How to avoid possible aggregation of magnetic beads during
storage or use?
A1: Beads should be stored at 2~8℃, and avoid for irreversible
aggregation due to contamination or drying. Magnetic beads
aggregation at low pH elution buffer is a normal phenomenon, and it
does not affect the normal use of magnetic beads. In
Binding/Washing buffer and Elution buffer adding with the final
concentration of 0.1% (v/v) nonionic detergent (such as NP-40,
Tween-20 or Triton X-100) can effectively prevent the beads
aggregation. The magnetic beads in low pH elution can be washed to
neutral with Binding/Washing buffer and Elution buffer, and process
with 2 min ultrasonic water bath. It can restore the magnetic beads
to homogeneity, and the above treatments had no effect on the
antibody binding efficiency with magnetic beads.
Q2: How to solve magnetic bead adhere to tube wall?
A2: It is recommended to use the consumables with low adsorption
rate. In addition, adding 0.01%~0.1%(v/v) nonionic detergent (such
as NP-40, Tween-20 or Triton X-100) to the buffer solution can
effectively reduce the adhesion of beads on tube wall.
