Magrose Protein A Magbeads for Protein Purification 30-150 μm, 10%
(v/v)
1, Description:
This product is micron magnetic beads, skilled operation can
complete the antibody adsorption process within 15 min, and
complete the process of antibody purification within 30 min. The
one-step solution can reduce the antibody absorption process to 10
minutes and purification process to 30 minutes, yielding antibody
with >90% purity. This product can be used repeatedly, which is
suitable for the purification of antibodies in plasma, ascites,
tissue culture supernatant and other samples.
2, Product Information:
| Product Name | Magrose Protein A |
Bead Size | 30~150 μm |
Beads Concentration | 10% (v/v) |
Ligand | Protein A |
Medium | Magrose |
Antibody Bonding Quantity | 25~30 mg Human IgG/mL Gel |
Storage Temperature | 2~8℃ |
| Binding/Washing Buffer | PBST (pH 7.2~7.4): 137 mM NaCl,2.7 mM KCl, 10 mM Na2HPO4, 2.0 mM KH2PO4, 0.1% Tween-20 |
Elution buffer | 100 mM Gly, 0.1% Tween-20, pH 2.5 |
Neutrilization buffer | 1.0 M Tris-HCl, pH 9.0 |
Storage buffer | PBST (includes 0.05% NaN3) |
Shelf life | 12 months |
3, Feature:
1. Highly effective antibody binding capacity and ultra-low
non-specific adsorption performance
2. Time saving, simple and mild
3. High product stability
4. Antibody elution system is closer to neutral
5. Very low protein A ligand off rate
6. Reusable
4, FAQ:
Q1: How to improve the efficiency of antibody elution?
A1: When the high affinity between antibody and Protein A ligand
result in antibody low elution efficiency, users can avoid this
situation by reducing the pH value (1.9~2.5) of elution buffer,
increasing the ionic strength of elution buffer (2~3 M MgCl2) or
prolonging the elution time. But pay attention that antibody could
easily aggregate under low pH conditions. Users can adjust the pH
to neutral immediately with alkaline buffers (such as Tris, HEPES,
etc.).
Q2: How to avoid possible aggregation of magnetic beads during
storage or use?
A2: Beads should be stored at 2~8℃, and avoid for irreversible
aggregation due to contamination or drying. Magnetic beads
aggregation at low pH elution buffer is a normal phenomenon, and it
does not affect the normal use of magnetic beads. In
Binding/Washing buffer and Elution buffer adding with the final
concentration of 0.1% (v/v) nonionic detergent (such as NP-40,
Tween-20 or Triton X-100) can effectively prevent the beads
aggregation. The magnetic beads in low pH elution can be washed to
neutral with Binding/Washing buffer and Elution buffer, and process
with 2 min ultrasonic water bath. It can restore the magnetic beads
to homogeneity, and the above treatments had no effect on the
antibody binding efficiency with magnetic beads.
