Magrose Protein G Magbeads for Protein Purification 30-150 μm, 10%
(v/v)
1, Description:
This product is micron magnetic beads, skilled operation can
complete the antibody adsorption process within 15 min, and
complete the process of antibody purification within 30 min. The
one-step solution can reduce the antibody absorption process to 10
minutes and purification process to 30 minutes, yielding antibody
with >90% purity. It can be used for antibody fixation and other
related studies. By referring the attached Table 1 users can select
the categories of magnetic beads according to the source and
subtype of the target antibody, and compare the affinity of Magrose
Protein A and Magrose Protein G beads with different antibodies.
2, Product Information:
| Product Name | Magrose Protein A |
Bead Size | 30~150 μm |
Beads Concentration | 10% (v/v) |
Ligand | Protein A |
Medium | Magrose |
Antibody Bonding Quantity | 25~30 mg Human IgG/mL Gel |
Storage Temperature | 2~8℃ |
| Binding/Washing Buffer | PBST (pH 7.2~7.4): 137 mM NaCl,2.7 mM KCl, 10 mM Na2HPO4, 2.0 mM KH2PO4, 0.1% Tween-20 |
Elution buffer | 100 mM Gly, 0.1% Tween-20, pH 2.5 |
Neutrilization buffer | 1.0 M Tris-HCl, pH 9.0 |
Storage buffer | PBST (includes 0.05% NaN3) |
Shelf life | 12 months |
3, Feature:
1. Highly effective antibody binding capacity and ultra-low
non-specific adsorption performance
2. Time saving, simple and mild
3. High product stability
4. Antibody elution system is closer to neutral
5. Very low protein A ligand off rate
6. Reusable
4, FAQ:
Q1: How to avoid possible aggregation of magnetic beads during
storage or use?
A1: Beads should be stored at 2~8℃, and avoid for irreversible
aggregation due to contamination or drying. Magnetic beads
aggregation at low pH elution buffer is a normal phenomenon, and it
does not affect the normal use of magnetic beads. In
Binding/Washing buffer and Elution buffer adding with the final
concentration of 0.1% (v/v) nonionic detergent (such as NP-40,
Tween-20 or Triton X-100) can effectively prevent the beads
aggregation. The magnetic beads in low pH elution can be washed to
neutral with Binding/Washing buffer and Elution buffer, and process
with 2 min ultrasonic water bath. It can restore the magnetic beads
to homogeneity, and the above treatments had no effect on the
antibody binding efficiency with magnetic beads.
Q2: Magnetic beads appear agglomeration in the process of using?
A2: The agglomeration phenomenon is generally difficult to shake
and break, which easily lead to uneven distribution. The reason is
mainly because the magnetic beads are placed in the magnetic field
for too long, the magnetic beads firmly binding together. The
magnetic beads can be dispersed by 2 min ultrasonic water bath, but
it should be noted that the antibody captured in the sample
solution can lost from ultrasonic treatment, so it’s not
recommended to use this method until elution.
