Magrose Protein G Magbeads for Protein Purification 30-150 μm, 10%
(v/v)
1, Description:
BeaverBeads™ Protein A, Protein G are superparamagnetic beads
covalently coated with recombinant Protein A or Protein G that
contains Fc binding domains for the purification of monoclonal or
polyclonal antibodies from cell lysate, blood plasma, ascites and
tissue culture supernatant, and for antibody immobilization
studies. This product can be used repeatedly, which is suitable for
the purification of antibodies in plasma, ascites, tissue culture
supernatant and other samples. It can also be used for antibody
fixation and other related studies. By referring the attached Table
1 users can select the categories of magnetic beads according to
the source and subtype of the target antibody, and compare the
affinity of Magrose Protein A and Magrose Protein G beads with
different antibodies.
2, Product Information:
| Product Name | Magrose Protein A |
Bead Size | 30~150 μm |
Beads Concentration | 10% (v/v) |
Ligand | Protein A |
Medium | Magrose |
Antibody Bonding Quantity | 25~30 mg Human IgG/mL Gel |
Storage Temperature | 2~8℃ |
| Binding/Washing Buffer | PBST (pH 7.2~7.4): 137 mM NaCl,2.7 mM KCl, 10 mM Na2HPO4, 2.0 mM KH2PO4, 0.1% Tween-20 |
Elution buffer | 100 mM Gly, 0.1% Tween-20, pH 2.5 |
Neutrilization buffer | 1.0 M Tris-HCl, pH 9.0 |
Storage buffer | PBST (includes 0.05% NaN3) |
Shelf life | 12 months |
3, Feature:
1. Highly effective antibody binding capacity and ultra-low
non-specific adsorption performance
2. Time saving, simple and mild
3. High product stability
4. Antibody elution system is closer to neutral
5. Very low protein A ligand off rate
6. Reusable
4, FAQ:
Q1: How to improve the efficiency of antibody elution?
A1: When the high affinity between antibody and Protein A ligand
result in antibody low elution efficiency, users can avoid this
situation by reducing the pH value (1.9~2.5) of elution buffer,
increasing the ionic strength of elution buffer (2~3 M MgCl2) or
prolonging the elution time. But pay attention that antibody could
easily aggregate under low pH conditions. Users can adjust the pH
to neutral immediately with alkaline buffers (such as Tris, HEPES,
etc.).
Q2: How to solve magnetic bead adhere to tube wall?
A2: It is recommended to use the consumables with low adsorption
rate. In addition, adding 0.01%~0.1%(v/v) nonionic detergent (such
as NP-40, Tween-20 or Triton X-100) to the buffer solution can
effectively reduce the adhesion of beads on tube wall.
