Magrose Protein G Magbeads for Protein Purification 30-150 μm, 10%
(v/v)
1, Description:
This product is micron magnetic beads, skilled operation can
complete the antibody adsorption process within 15 min, and
complete the process of antibody purification within 30 min. This
product can be used repeatedly, which is suitable for the
purification of antibodies in plasma, ascites, tissue culture
supernatant and other samples. It can also be used for antibody
fixation and other related studies. By referring the attached Table
1 users can select the categories of magnetic beads according to
the source and subtype of the target antibody, and compare the
affinity of Magrose Protein A and Magrose Protein G beads with
different antibodies.
2, Product Information:
| Product Name | Magrose Protein A |
Bead Size | 30~150 μm |
Beads Concentration | 10% (v/v) |
Ligand | Protein A |
Medium | Magrose |
Antibody Bonding Quantity | 25~30 mg Human IgG/mL Gel |
Storage Temperature | 2~8℃ |
| Binding/Washing Buffer | PBST (pH 7.2~7.4): 137 mM NaCl,2.7 mM KCl, 10 mM Na2HPO4, 2.0 mM KH2PO4, 0.1% Tween-20 |
Elution buffer | 100 mM Gly, 0.1% Tween-20, pH 2.5 |
Neutrilization buffer | 1.0 M Tris-HCl, pH 9.0 |
Storage buffer | PBST (includes 0.05% NaN3) |
Shelf life | 12 months |
3, Feature:
4, FAQ:
1. Highly effective antibody binding capacity and ultra-low
non-specific adsorption performance
2. Time saving, simple and mild
3. High product stability
4. Antibody elution system is closer to neutral
5. Very low protein A ligand off rate
6. Reusable
Q1: How to improve the binding efficiency of antibody with magnetic
beads?
A1: The binding efficiency of antibody with magnetic beads is
related to the origin of the antibody and its subtype, refer to the
type of antibody and the affinity efficiency of Protein A ligand
antibody. (See attached table 1 ). If the antibody subtype has low
affinity with Protein A , users can increase incubation time
between antibody and beads (30~120 min), increase pH value (8~9) of
the combination buffer and decrease ionic strength (25~100 mM NaCl)
to increase affinity efficiency, or select the target antibodies
with higher affinity ligands (such
as Protein G or Protein A/G).
Q2: How to solve magnetic bead adhere to tube wall?
A2: It is recommended to use the consumables with low adsorption
rate. In addition, adding 0.01%~0.1%(v/v) nonionic detergent (such
as NP-40, Tween-20 or Triton X-100) to the buffer solution can
effectively reduce the adhesion of beads on tube wall.
