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Brand Name : | BeaverBeads |
Model Number : | 70808-50 |
Certification : | COA |
Price : | Negotiation |
Payment Terms : | L/C, T/T |
Supply Ability : | 850 L/month |
Delivery Time : | 5-8 days |
Agarose Strep-tag Ⅱ Protein Purification Magbeads 30-150 μm, 10% (v/v)
1, Description:
Strep-Tag system is a new type of protein purification system which simulates the streptavidin-biotin system. Compared to streptavidin, Strep-Tactin's affinity to Strep-Tag II is at least 10 times larger. The purification extraction condition of strep-tag is mild and the protein can be separated and purified under physiological conditions. In addition, compared to other tags, Strep-Tag II is small 8 amino acid tag (WSHPQFEK). Since the tag is small, it is only about 1 kDa which doesn't affect the structure and function of the protein after fusion. These mild purification condition preserve the biological activity of the protein and produce more than 99% purity in only one extraction step.
2, Product Information:
Product Name | Agarose Strep Tactin Magbeads |
Bead Size | 30~150 μm |
Ligand density | ~6 mg Strep-Tactin / mL Gel |
Protein binding Capacity (mg/ml pure beads) | ~7 mg Strep-tag Ⅱprotein / mL Gel |
Suspension concentration | 10%(v/v)magnetic beads suspension |
Binding Buffer | 50 mM Tris-HCl, pH 8.0 |
Elution Buffer | 2.5 mM desthiobiotin in Binding Buffer |
Storage buffer | 1×PBS (0.1% Tween-20+0.05% NaN3) |
Shelf life | 2 years when stores at 2 ~ 8 ℃, |
Regeneration Buffer | 0.5 M NaOH or 1 mM HABA in Binding Buffer |
3, Features:
1. High capability of antibody binding.
2. Time-saving, simple and mild.
3. Obtained high-purity protein by one-Step.
4. No need removing the tag.
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