Agarose NHS Magbeads Preactivated N - Hydroxy Succinimide
Nanoparticle 10 - 30 μm, 20% (v/v)
1, Description:
The NHS magnetic beads are superparamagnetic beads with activated
groups of N_Hydroxysuccinimide on the surface, which can react with
primary amines on desired ligands and bind them covalently with
amide linkage to the beads. The ligand can be various molecules
including antigen, antibody and other proteins. Compared with the
traditional carboxyl, amino magnetic beads, the NHS group of
magnetic beads don’t need to activate EDC/NHS or Glutaraldehyde,
simply dissolving the amino-containing bio-ligand in the coupling
buffer, at room temperature, mixing with proteins and the NHS
magnetic beads for only 1-2 hours, the bio-ligand can be coupled to
the magnetic beads. The advantages include easy operation, mild
coupling conditions and coupling fast and efficient. The magnetic
bead coupling process must be carried out in a buffer solvent free
of any amino. This method can be operated manually or
automatically.
2, Introduction:
| Product Name | Agarose NHS Magbeads Preactivated |
| Mean particle size | 10-30 μm |
| Magnetic core | Fe3O4 |
| Shell | Agarose |
| Magnetic | superparamagnetism |
| Ligand content | ~50 μM/mL gel |
| Binding ability | 20~30 mg rabbit IgG/mL beads |
| Preservation fluid | 20% (v/v) ethanol solution |
| Operation | Automate/manual |
| * water average particle size, Malvern Nano determination |
3, FEATURES:
1. Simple, without activation, can be directly covalently coupled
with the biological ligand;
2. Efficient, bio-ligand coupling efficiency of up to 90% or more,
much higher than the carboxyl beads, while the ligand with a higher
binding capacity;
3. Fast, 1 ~ 2h to complete the bio-ligand coupling;
4. Mild, room temperature or 4 ℃ coupling, coupling system pH 5 ~
9;
5. Stable, the formation of a stable amide bond, to prevent the
ligand off.
6. Good biocompatibility, reduced nonspecific adsorption.
4, Precautions:
1. Magnetic beads are sensitive to water. In order to ensure
product quality, after sampling immediately cover
the bottle caps and seal with a sealing tape, store at 4 ℃.
2. Protein stabilizers (such as BSA, gelatin) inhibit the binding
of antibodies to magnetic beads. Therefore,
during the bead-coupled antibody process, it is necessary to ensure
that no primary amino-containing
protein stabilizer is present in the antibody storage system.
3. The presence of a primary amine-containing substance in the
buffer inhibits protein coupled with the beads.
Removal of primary amine materials can use dialysis and
desalination methods.
