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Agarose NHS Magnetic Beads Preactivated N - Hydroxy Succinimide Nanoparticle

    Buy cheap Agarose NHS Magnetic Beads Preactivated N - Hydroxy Succinimide Nanoparticle from wholesalers
     
    Buy cheap Agarose NHS Magnetic Beads Preactivated N - Hydroxy Succinimide Nanoparticle from wholesalers
    • Buy cheap Agarose NHS Magnetic Beads Preactivated N - Hydroxy Succinimide Nanoparticle from wholesalers

    Agarose NHS Magnetic Beads Preactivated N - Hydroxy Succinimide Nanoparticle

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    Brand Name : BeaverBeads
    Model Number : 70705-50
    Certification : COA
    Price : Negotiation
    Payment Terms : T/T, L/C
    Supply Ability : 850 L/month
    Delivery Time : 5-8 days
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    Agarose NHS Magnetic Beads Preactivated N - Hydroxy Succinimide Nanoparticle

    Agarose NHS Magbeads Preactivated N - Hydroxy Succinimide Nanoparticle​ 10 - 30 μm, 20% (v/v)


    1, Description:

    The NHS magnetic beads are superparamagnetic beads with activated groups of N_Hydroxysuccinimide on the surface, which can react with primary amines on desired ligands and bind them covalently with amide linkage to the beads. The ligand can be various molecules including antigen, antibody and other proteins. Compared with the traditional carboxyl, amino magnetic beads, the NHS group of magnetic beads don’t need to activate EDC/NHS or Glutaraldehyde, simply dissolving the amino-containing bio-ligand in the coupling buffer, at room temperature, mixing with proteins and the NHS magnetic beads for only 1-2 hours, the bio-ligand can be coupled to the magnetic beads. The advantages include easy operation, mild coupling conditions and coupling fast and efficient. The magnetic bead coupling process must be carried out in a buffer solvent free of any amino. This method can be operated manually or automatically.
    The biological ligand can be covalently coupled to the magnetic beads. It has the advantages of simple operation, mild coupling condition and quick and efficient coupling of biological ligands. The difference between BeaverBeads ™ Mag NHS and BeaverBeads ™ Magrose NHS is that Mag NHS is a polymer-based solid microsphere with a particle size of 2 μm and is mainly used for analytical areas (eg in vitro diagnostics, microbiological testing, immunoprecipitation, immunization Precipitation, etc.); and Magrose NHS is a natural hydrophilic polymer agarose as the substrate of porous microspheres, particle size of 30 ~ 100μm, which has good biocompatibility, a larger surface area and biological Based coupling, mainly used for separation and purification of the field (such as protein separation and purification, antibody isolation and purification).

    2, Introduction:

    Product NameAgarose NHS Magbeads Preactivated
    Mean particle size10-30 μm
    Magnetic coreFe3O4
    ShellAgarose
    Magneticsuperparamagnetism
    Ligand content~50 μM/mL gel
    Binding ability20~30 mg rabbit IgG/mL beads
    Preservation fluid20% (v/v) ethanol solution
    OperationAutomate/manual
    * water average particle size, Malvern Nano determination

    3, FEATURES:

    • 1. Simple, without activation, can be directly covalently coupled with the biological ligand;

    • 2. Efficient, bio-ligand coupling efficiency of up to 90% or more, much higher than the carboxyl beads, while the ligand with a higher binding capacity;

    • 3. Fast, 1 ~ 2h to complete the bio-ligand coupling;

    • 4. Mild, room temperature or 4 ℃ coupling, coupling system pH 5 ~ 9;

    • 5. Stable, the formation of a stable amide bond, to prevent the ligand off.

    • 6. Good biocompatibility, reduced nonspecific adsorption.

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