Protein A Antibody Purification Kit for Protein Purification 2μm, 30 mg / mL
1, Description:
This product is micron magnetic beads, skilled operation can
complete the antibody adsorption process within 15 min, and
complete the process of antibody purification within 30 min. The
magnetic bead antibody purification kit is equipped with optimized
prefabricated buffer, which provides the best reaction conditions
for the antibody purification experiment and enhances its
stability. This product can be used repeatedly, which is
suitable for the purification of antibodies in plasma, ascites,
tissue culture supernatant and other samples. It can also be used
for antibody fixation and other related studies. By referring the
attached Table 1 users can select the categories of magnetic beads
according to the source and subtype of the target antibody, and
compare the affinity of Magrose Protein A and Magrose Protein G
beads with different antibodies.
2, Product Information:
| Product Name | BeaverBeadsTM Protein A(or A/G)Antibody Purification Kit |
Protein A(or A/G)for Antibody Purification ① | 1 mL |
Antibody Binding Buffer ② | 100 mL |
Antibody Elution Buffer I(pH4.5)③ | 50 mL |
Antibody Elution Buffer II(pH2.0)④ | 50 mL |
Antibody Neutrilization buffer ⑤ | 10 mL |
Beads Washing Buffer ⑥ | 10 mL |
Beads Storage Buffer ⑦ | 10 mL |
Beads Regeneration Buffer ⑧ | 10 mL |
Storage Conditions | 2~8℃ for long-term preservation |
Shelf life | 2 year |
3, Feature:
1. No centrifugation, magnetic separation can be used, and the use
of the chromatograph-agarose gel purification system can greatly
shorten the operating time
2. Avoid the error of the packing loss caused by repeated suction
fluid
3. Avoid damage to active protein by mechanical shearing force in
centrifugation
4, FAQ:
Q1: How to improve the binding efficiency of antibody with magnetic
beads?
A1: The binding efficiency of antibody with magnetic beads is
related to the origin of the antibody and its subtype, refer to the
type of antibody and the affinity efficiency of Protein A ligand
antibody. (See attached table 1 ). If the antibody subtype has low
affinity with Protein A , users can increase incubation time
between antibody and beads (30~120 min), increase pH value (8~9) of
the combination buffer and decrease ionic strength (25~100 mM NaCl)
to increase affinity efficiency, or select the target antibodies
with higher affinity ligands (such as Protein G or Protein A/G).
Q2: How to improve the efficiency of antibody elution?
A2: When the high affinity between antibody and Protein A ligand
result in antibody low elution efficiency, users can avoid this
situation by reducing the pH value (1.9~2.5) of elution buffer,
increasing the ionic strength of elution buffer (2~3 M MgCl2) or
prolonging the elution time. But pay attention that antibody could
easily aggregate under low pH conditions. Users can adjust the pH
to neutral immediately with alkaline buffers (such as Tris, HEPES,
etc.).
