Protein A Antibody Purification Kit for Protein Purification 2μm, 30 mg / mL
1, Description:
BeaverBeadsTM Protein A(or Protein G) antibody purification beads are NHS
activated superparamagnetic beads covalent binding Protein A (or
Protein G). Compared with the similar products in the international
market, the product has higher antibody binding capacity and lower
protein non-specific adsorption rate, and the elution conditions
are more homogeneous. The antibody can be separated from the serum
sample by one-step purification, and the purity of the antibody is
more than 90%.
2, Product Information:
| Product Name | BeaverBeadsTM Protein A(or A/G)Antibody Purification Kit |
Protein A(or A/G)for Antibody Purification ① | 1 mL |
Antibody Binding Buffer ② | 100 mL |
Antibody Elution Buffer I(pH4.5)③ | 50 mL |
Antibody Elution Buffer II(pH2.0)④ | 50 mL |
Antibody Neutrilization buffer ⑤ | 10 mL |
Beads Washing Buffer ⑥ | 10 mL |
Beads Storage Buffer ⑦ | 10 mL |
Beads Regeneration Buffer ⑧ | 10 mL |
Storage Conditions | 2~8℃ for long-term preservation |
Shelf life | 2 year |
3, Feature:
1. No centrifugation, magnetic separation can be used, and the use
of the chromatograph-agarose gel purification system can greatly
shorten the operating time
2. Avoid the error of the packing loss caused by repeated suction
fluid
3. Avoid damage to active protein by mechanical shearing force in
centrifugation
4, FAQ:
Q1: How to improve the binding efficiency of antibody with magnetic
beads?
A1: The binding efficiency of antibody with magnetic beads is
related to the origin of the antibody and its subtype, refer to the
type of antibody and the affinity efficiency of Protein A ligand
antibody. (See attached table 1 ). If the antibody subtype has low
affinity with Protein A , users can increase incubation time
between antibody and beads (30~120 min), increase pH value (8~9) of
the combination buffer and decrease ionic strength (25~100 mM NaCl)
to increase affinity efficiency, or select the target antibodies
with higher affinity ligands (such as Protein G or Protein A/G).
Q2: How to avoid possible aggregation of magnetic beads during
storage or use?
A2: Beads should be stored at 2~8℃, and avoid for irreversible
aggregation due to contamination or drying. Magnetic beads
aggregation at low pH elution buffer is a normal phenomenon, and it
does not affect the normal use of magnetic beads. In
Binding/Washing buffer and Elution buffer adding with the final
concentration of 0.1% (v/v) nonionic detergent (such as NP-40,
Tween-20 or Triton X-100) can effectively prevent the beads
aggregation. The magnetic beads in low pH elution can be washed to
neutral with Binding/Washing buffer and Elution buffer, and process
with 2 min ultrasonic water bath. It can restore the magnetic beads
to homogeneity,
and the above treatments had no effect on the antibody binding
efficiency with magnetic beads.
