Sign In | Join Free | My himfr.com
Home > NHS Activated Magnetic Beads >

2 μm Polymer NHS Magbeads Preactivated 10 mg/mL 50 mL

    Buy cheap 2 μm Polymer NHS Magbeads Preactivated 10 mg/mL 50 mL from wholesalers
     
    Buy cheap 2 μm Polymer NHS Magbeads Preactivated 10 mg/mL 50 mL from wholesalers
    • Buy cheap 2 μm Polymer NHS Magbeads Preactivated 10 mg/mL 50 mL from wholesalers

    2 μm Polymer NHS Magbeads Preactivated 10 mg/mL 50 mL

    Ask Lasest Price
    Brand Name : BeaverBeads
    Model Number : 70701-50
    Certification : COA
    Price : Negotiation
    Payment Terms : T/T, L/C
    Supply Ability : 850 L/month
    Delivery Time : 5-8 days
    • Product Details
    • Company Profile

    2 μm Polymer NHS Magbeads Preactivated 10 mg/mL 50 mL

    Polymer NHS Magbeads Preactivated N - Hydroxy Succinimide Nanoparticle​ 2 μm, 10 mg/mL


    1, Description:

    The NHS magnetic beads are superparamagnetic beads with activated groups of N_Hydroxysuccinimide on the surface, which can react with primary amines on desired ligands and bind them covalently with amide linkage to the beads. The ligand can be various molecules including antigen, antibody and other proteins. Compared with the traditional carboxyl, amino magnetic beads, the NHS group of magnetic beads don’t need to activate EDC/NHS or Glutaraldehyde, simply dissolving the amino-containing bio-ligand in the coupling buffer, at room temperature, mixing with proteins and the NHS magnetic beads for only 1-2 hours, the bio-ligand can be coupled to the magnetic beads. The advantages include easy operation, mild coupling conditions and coupling fast and efficient. The magnetic bead coupling process must be carried out in a buffer solvent free of any amino. This method can be operated manually or automatically.

    2, Introduction:

    Product NamePolymer NHS Magbeads Preactivated
    Mean particle size2 μm
    Magnetic coreFe3O4
    ShellPolymer
    Magneticsuperparamagnetism
    Ligand content~50 μM/mL gel
    Binding ability20~30 mg rabbit IgG/mL beads
    Preservation fluidDMAC
    OperationAutomate/manual
    * water average particle size, Malvern Nano determination

    3, FEATURES:

    • 1. Simple, without activation, can be directly covalently coupled with the biological ligand;

    • 2. Efficient, bio-ligand coupling efficiency of up to 90% or more, much higher than the carboxyl beads, while the ligand with a higher binding capacity;

    • 3. Fast, 1 ~ 2h to complete the bio-ligand coupling;

    • 4. Mild, room temperature or 4 ℃ coupling, coupling system pH 5 ~ 9;

    • 5. Stable, the formation of a stable amide bond, to prevent the ligand off.

    • 6. Good biocompatibility, reduced nonspecific adsorption.


    4, Precautions:

    1. Magnetic beads are sensitive to water. In order to ensure product quality, after sampling immediately cover
    the bottle caps and seal with a sealing tape, store at 4 ℃.
    2. Beads are not allowed to dry or freeze. Drying and freezing operations may result in the aggregation of
    magnetic beads and thus loss of binding activity.
    3. Pre-and post-reaction protein content can be determined by indirect methods (e.g., Thermo Scientific ™
    Pierce ™ 660 nm Protein Assay, Product No. 22660 and 22662) or by direct methods (e.g., use the Thermo
    Scientific ™ Pierce ™ Micro BCA Protein Assay, Product No. 23235) to detect the surface of the magnetic
    beads coupled with the protein content. Using wavelengths around 280 nm to determine protein levels is
    not advisable. Because NHS groups strongly absorb near 280nm, they can seriously interfere with the
    detection.


    Quality 2 μm Polymer NHS Magbeads Preactivated 10 mg/mL 50 mL for sale
    Inquiry Cart 0
    Send your message to this supplier
     
    *From:
    *To: BEAVER Biomedical Engineering Co., LTD.
    *Subject:
    *Message:
    Characters Remaining: (0/3000)