Polymer NHS Magbeads Preactivated N - Hydroxy Succinimide
Nanoparticle 2 μm, 10 mg/mL
1, Description:
BeaverBeads ™ Mag NHS and BeaverBeads ™ Magrose NHS are spherical
beads modified with NHS groups capable of forming stable peptide
bonds with proteins and other molecules with primary amine groups
for affinity purification or assay detection of antibodies,
antigens and other biomolecules. Compared with the traditional
beads with carboxyl or amino groups, the beads with surface
containing NHS group do not need to be activated by EDC / NHS or
glutaraldehyde in advance. Simply by dissolving the ligand
containing the primary amino acid in the coupling buffer (mix the
protein solution with Mag NHS beads at room temperature for 1 ~
2h), the ligands will be attached on the beads.
2, Introduction:
| Product Name | Polymer NHS Magbeads Preactivated |
| Mean particle size | 2 μm |
| Magnetic core | Fe3O4 |
| Shell | Polymer |
| Magnetic | superparamagnetism |
| Ligand content | ~50 μM/mL gel |
| Binding ability | 20~30 mg rabbit IgG/mL beads |
| Preservation fluid | DMAC |
| Operation | Automate/manual |
| * water average particle size, Malvern Nano determination |
3, FEATURES:
1. Simple, without activation, can be directly covalently coupled
with the biological ligand;
2. Efficient, bio-ligand coupling efficiency of up to 90% or more,
much higher than the carboxyl beads, while the ligand with a higher
binding capacity;
3. Fast, 1 ~ 2h to complete the bio-ligand coupling;
4. Mild, room temperature or 4 ℃ coupling, coupling system pH 5 ~
9;
5. Stable, the formation of a stable amide bond, to prevent the
ligand off.
6. Good biocompatibility, reduced nonspecific adsorption.
4, Precautions:
1. Magnetic beads are sensitive to water. In order to ensure
product quality, after sampling immediately cover the bottle caps
and seal with a sealing tape, store at 4 ℃.
2. Beads are not allowed to dry or freeze. Drying and freezing
operations may result in the aggregation of magnetic beads and thus
loss of binding activity.
3. Pre-and post-reaction protein content can be determined by
indirect methods (e.g., Thermo Scientific™ Pierce™ 660 nm Protein
Assay, Product No. 22660 and 22662) or by direct methods (e.g., use
the Thermo Scientific™ Pierce™ Micro BCA Protein Assay, Product No.
23235) to detect the surface of the magnetic beads coupled with the
protein content. Using wavelengths around 280 nm to determine
protein levels is
not advisable. Because NHS groups strongly absorb near 280nm, they
can seriously interfere with the detection.
